Early Detection Of Human Papilloma And Other Viral Infections
17.10.2007 08:41 Science - Source: ScienceDaily Headlines
In the study, Edward S. Yeung and colleagues point out that the most sensitive existing test for viral infections has drawbacks. That test is the Nobel Prize-winning polymerase chain reaction (PCR), used to detect DNA in settings ranging from medical labs to crime scenes. PCR requires an initial step in which scientists "amplify," or copy, a DNA sample a thousand-fold before virus detection can begin.
However, amplification increases the risk of false-positives and false-negatives, especially when a sample has even a tiny amount of contaminants. Since over 50 million Pap smears are performed in the United States each year to test for HPV -- the leading cause of cervical cancer -- a fast, simple, accurate diagnosis is essential.
The new method skips the amplification step entirely, and yet can detect the presence of less than two copies of HPV per cell -- a level corresponding to very early infection. The technique, called single-molecule spectroscopy, could be easily integrated into the Pap smear method. "It can become a good clinical screening or quantification method for viral DNA in cells," opening the door to improved screening tests for hepatitis B, herpes and other diseases.
Article: "Single-Molecule Detection of Surface-Hybridized Human Papilloma Virus DNA for Quantitative Clinical Screening" Nov. 1 issue of Analytical Chemistry.
Note: This story has been adapted from material provided by American Chemical Society.
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